Analytical method validation for quantitative estimation of chloramben by HPLC for assay of amiben’ DS chloramben herbicide

Abstract

A simple, selective, precise and accurate High Performance liquid Chromatographic method for the analysis of
Chloramben in its formulations was developed and validated in the present study. The mobile phase consist a mixture of
0.05% H3PO4 solution and acetonitrile in the proportion 20: 80 (v/v). This was found to give sharp peak of Chloramben at
a run time of 15 min. HPLC analysis of Chloramben was carried out at a wave length of 225 nm with a flow rate of
1.0mL/ min. The linear regression analysis data for the calibration curve showed a good linear relationship with a
regression coefficient 0.999 in the concentration range of 50% to 150%. The linear regression equation was y =3476.7 x -
41.412. The developed method was employed with a high degree of precision and accuracy for the analysis of
Chloramben. The method was validated for accuracy, precision, robustness, ruggedness and specificity. The Precision,
accuracy, sensitivity, short retention time and composition of the mobile phase indicated that this method is useful for the
quantification of Chloramben.