RP-HPLC Method Development and Validation for the Simultaneous Estimation of Atenolol and Indapamide in Pharmaceutical Tablet Dosage Form

Abstract

A simple reversed-phase high-performance liquid chromatographic (RP-HPLC) method has been developed and
validated for simultaneous determination of ATENOLOL and INDAPAMIDE in pharmaceutical tablet dosage form.
Chromatographic analysis was performed on a Symmetry X-terra C8 (4.6mm x 100mm, 5m) column at ambient
temperature with a mixture of Potassium di hydrogen phosphate buffer and Acetonitrile in the ratio 40:60 v/v as
mobile phase, at a flow rate of 0.7 mL min-1
. UV detection was performed at 240 nm. The retention times of
Atenolol and Indapamide were 2.1 and 3.6 min, respectively. The correlation coefficient of Atenolol and
Indapamide was found to be 0.999. Calibration plots were linear over the concentration ranges 20–100 μg mL- 1 and
1-5 μg mLfor Atenolol and Indapamide respectively. The Limit of detection was 0.223 and 0.286µg mL-1
and the
quantification limit were 0.677 µg mL-1
and 0.86µg mL-1 for Atenolol and Indapamide, respectively. The accuracy
of the proposed method was determined by recovery studies and found to be 100.74% to 99.93%.The method was
validated for accuracy, linearity, sensitivity, precision, robustness, system suitability Commercial tablet formulation
was successfully analyzed using the developed method and the proposed method is applicable to routine analysis of
determination of Atenolol and Indapamide in pharmaceutical tablet dosage form.