Development and validation of stability-indicating method for the estimation of pyrimethamine in tablet dosage form

Authors

  • Kannan Jakkan Department of Chemistry, Jaipur National University, Rajashthan. India
  • Nataraj Palaniappan Novitium Pharma LLC, New jersey, USA.
  • Ravisankar Mathesan Vinayaka missions Research foundation, Salem.India.

DOI:

https://doi.org/10.61096/ijpar.v9.iss3.2020.143-149

Keywords:

RP- HPLC, Pyrimethamine, Method development, Validation

Abstract

A novel HPLC method was developed with precise, accurate, linear robust, rugged and validated as per ICH guidelines by using high performance liquid chromatography method used for the analysis of pyrimethamine in pharmaceutical dosage form. The HPLC method has been carried out by using Agilent zorbax SB -C8, 150 x 4.6 mm 3.5μm column. The mobile phase consist of pH 4.0 buffer: methanol in the ratio of 55:45 %v/v and the flow rate of 1.2 ml/min by the detection of UV at 280 nm. The retention time of the pyrimethamine is 4.98 min. Total chromatography runtime is 12 min. The linearity range w as found to be over a concentration of 60% -160% with a correlation coefficient of 0.9999.The accuracy was found to be 99.4 to 100.7%.The projected method can be utilised for the analysis of the drug in pharmaceutical formulation.

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Published

2020-09-17

How to Cite

Kannan Jakkan, Nataraj Palaniappan, & Ravisankar Mathesan. (2020). Development and validation of stability-indicating method for the estimation of pyrimethamine in tablet dosage form. IJPAR JOURNAL, 9(3), 143–149. https://doi.org/10.61096/ijpar.v9.iss3.2020.143-149

Issue

Vol. 9 No. 3 (2020): 2020 Volume - 9 Issue - 3

Section

Articles