Stability indicating spectrophotometric method for determination of brivudine

Abstract

A stability indicating spectrophotometric method was developed for the determination of brivudine in presence of its degradation products. Hence an attempt was made to develop stress degradation and stability indicating studies  of  Brivudine.  UV  Spectrophotometric  method  was  reported  for  the  estimation  of  Brivudine  in formulation. Characterization of degradation products of Brivudine photometric method by demonstration of different   degradation   behavior   has   been   performed   as   per   the   current   ICH   guidelines.   The   UV spectrophotometric method is found to be simple, specific, sensitive, reproducible and precise because of good recovery and low standard deviation values. Since the developed method shown no interference from the excipients like talc, magnesium stearate etc. these method can be used for the routine analysis of Brivudine from its dosage form. Brivudine absorbance was slightly increased by oxidation degradation study. Stress degradation studies on Brivudine as per ICH guidelines were illustrated. From the studies we can conclude, that the drug is stable under all stress degradation conditions because the λmax  should not change. But slight changes occur in thermal degradation due to the variation of % RSD. Hence the proposed method is suitable for the analysis of Brivudine.