Pharmacological activity of Curcumin-Bio-Enhancer loaded polymeric loaded nanoparticles: An invitro cell line study

Abstract

Clinical utility of curcumin in the treatment of cancer is restricted due to multi-drug resistance and metabolism via glucuronidation and sulfation in the liver and intestine. To overcome these limitations, we have used Curcumin and Bio-Enhancer (piperine, quercetin and silibinin) loaded polymeric loaded nanoparticles. Sulforhodamine B assay was used to evaluate the anti-cancer activity of Curcumin and Bio-Enhancer (piperine, quercetin and silibinin) loaded polymeric loaded nanoparticles in comparison with pure curcumin and positive control doxorubicin on Ovkar-3, HL60 and HEPG2 cancer cell line. Curcumin and Bio-Enhancer (piperine, quercetin and silibinin) loaded polymeric loaded nanoparticles displayed enhanced anti-cancer activity on Ovkar-3, HL60 and HEPG2 cancer cell lines than the pure curcumin. Enhanced anti-cancer activity of Curcumin and Bio-Enhancer (piperine, quercetin and silibinin) loaded polymeric loaded nanoparticles might be due to reversal of multi-drug resistance and synergistic enhancement of anti-cancer activity of curcumin by the bio-enhancers. The study concludes that the Curcumin and Bio-Enhancer (piperine, quercetin and silibinin) loaded polymeric loaded nanoparticles would significantly enhance the anti-cancer activity of curcumin in the treatment of various cancers. However, this pilot study data requires further validation using molecular level studies and clinical trials.