Evaluate the Disinfectant Activity of Some Commercial Preparations by Rideal -Walker Test.

Abstract

The aim of this study was to evaluate and compare  practically  achieved  disinfection  efficacy of some locally available disinfectants on surfaces and infectious microbiological  hospital waste. Three disinfectants were tested at concentrations recommended by manufacturers on rough and smooth surfaces that were contaminated experimentally   by  locally  circulating   isolates   of  methicillin-resistant Staphylococcus   aureus,   multi   drug- resistant Acinetobacter  baumannii, Klebsiella  pneumoniae, Enterobacter  aerogenes, Pseudomonas  aeruginosa strains,  standard  isolate  of Salmonella  typhi and Candida  albicans.  Reduction  in microbial  counts  before  and after surface disinfection  was expressed as log reduction. A very heavy microbial waste load was simulated by immersing culture plates with heavy microbial growth in disinfectants.  Daily, a sample of disinfectant was taken and subjected to Rideal-walker  test.