A label-free electrochemical protein sensor of perchloric acid doped polyaniline

Abstract

In this study, we developed a potentiometric based protein sensor utilizing the interactions between charged
functional moieties of a target protein-protein interaction and the complexation between PANI and dopant
molecules. The sensor response depended on the isoelectric point of the protein. However, this dependency may
be exploited to enhance specificity of protein sensors at specific pH dependence. The conducting polymer was
found to respond by changing potential in the presence of biomolecules, demonstrating a direct chemical to
electronic transduction method. The influence of polymer surface and morphology of finished films was also
studied. This study demonstrated a conducting polymer able to respond to proteins at physiological conditions,
in other words a step towards the integration of these materials into implantable sensing system. Additionally
this sensor also had better ability to recognize the specific protein-protein interaction.