Development and validation of RP – HPLC method for the estimation of Tylosin tartrate in pure and pharmaceutical formulation

Abstract

A simple, fast, precise, selective and accurate RP-HPLC method was developed and validated for the
simultaneous determination of tylosin tartrate from pharmaceutical formulation. Chromatographic separation
was achieved gradient on a phenomenex c18 column (250 x 4.6 mm, 5 µ particle size) using a mobile phase.
Acetonitrile and water in the ratio of 90:10.the flow rate was 1.5ml / min and effluent was detected at 292 nm.
The retention time of tylosin tartrate was found to be 2min. linearity was observed in the concentration range of
50 -250µg /ml. The method was validated according to ICH guidelines with respect to specificity, linearity,
accuracy, precision and robustness. The method developed can be used for the routine analysis of tylosin
tartrate.