Spectral analysis of whole plant ethanol extract of Blepharisrepens (vahl) roth and its fraction

Abstract

The whole plant of Blepharisrepens (vahl) Roth, have a great medicinal value. The aim of the present study was
to determine the chemical profile of ethanolic extract of Blepharisrepens (vahl) Rothand its fractions (by various
spectral analysis) which will be useful for its proper identification when therapeutically used. Spectral analysis
was done by different analytical methods such as UV, IR, GC-MS and HPTLC studies in ethanol extract (EE) of
Blepharisrepens (vahl) Rothand its fractions. In HPTLC analysis, ethanolic extract and its fractions showed the
presence of different types of compound with different RF values ranging from 0.01 to 1.05. The GC-MS
analysis showed the presence of terpenoids (mono), fatty acids such as hexadecanoic acid (CAS) palmitic acid,
heptadecanoic acid(CAS) palmitic acid, benzene dicarboxylic acid, cyclo heptasiloxane, Octadecene -1-ol,
octadecenalin ethanol extract and its fractions ofBlepharisrepens (vahl) Roth.