Development and validation of RP-HPLC method for simultaneous estimation of gliclazide and metformin in pure and tablet dosage form

Abstract

A simple, precise, accurate and rapid reverse phase high performance liquid chromatographic method was developed for the
simultaneous estimation of Gliclazide(GLZ) and Metformin(MET) in pure and tablet dosage form. The method was carried
on Phenomex (kromosil-250 mm × 4.6 mm, 5 μm) column with mobile phase comprising of phosphate buffer and methanol
in the ratio 60:40 v/v. Flow rate was adjusted to 1.0ml/min and effluents were monitored at 230 nm. The retention time
obtained for Gliclazide and Metformin was 5.0 and 3.2 mint respectively. The calibration curves were linear in the
concentration range of 10-100μg/ml for Gliclazide and Metformin 62.5-625μg/ml for. The developed method was validated
in accordance to ICH guidelines.