Validated stability indicating RP-HPLC method for the simultaneous determination of ofloxacin, ornidazole, clobetasol propionate, terbinafine hydrochloride, methyl paraben, propyl paraben in bulk and pharmaceutical dosage form

Abstract

A new simple, precise, accurate and selective RP-HPLC method has been developed and validated for simultaneous
estimation of Ofloxacin (OFL) ,Ornidazole (ORD), Terbinafine hydrochloride (TFH) , clobetasol propionate(CLP) , Methyl
paraben(MP) ,propyl paraben(PP) in bulk and pharmaceutical dosage forms. The method was carried out on a Zodiac C18
(250mm x 4.6mm, 5µm) column with a mobile phase consisting of Ortho phosphoric acid buffer, PH
2.5 and Acetonitrile in
the ratio (82:18v/v) and flow rate of 1ml/min .The detection was carried out at 255nm. The retention time for estimation of
ofloxacin (0.712min), ornidazole(1.933min), Terbinafine hydrochloride (7.302min), clobetasol propionate (9.224min),
Methyl paraben (4.074min), propyl paraben(7.926min) .The Linearity of proposed method was investigated in the range of
1-960 µg/ml with r2 value for ofloxacin (0.999), ornidazole(0.999), Terbinafine hydrochloride (0.999), clobetasol propionate
(0.998), Methyl paraben (0.998), propyl paraben(0.997) .The amount of drug estimated by the proposed method was found to
be in good agreement with label claim. The developed method was validated for precision, accuracy, sensitivity, robustness
and ruggedness. Hence it can be applied for routine analysis of titled drug in bulk and pharmaceutical formulations.