RP-HPLC method development and validation of doxycycline in bulk and tablet formulation

Authors

  • Jeyabaskaran.M
  • Rambabu.C
  • Sree Janardhanan V
  • Rajinikanth.V
  • Pranitha.T
  • Dhanalakshmi.B

DOI:

https://doi.org/10.61096/ijpar.v3.iss4.2014.397-404

Keywords:

Doxycycline, RP-HPLC, Validation, method development

Abstract

A Simple, accurate and rapid RP-HPLC method has been developed for the estimation of doxycycline (DOXY) in bulk and
pharmaceutical dosage forms using an Altima C 18, 150 x 4.6 mm i.d, 5 µm particle size in isocratic mode; with mobile
phase comprising of buffer (0.1% of potassium dihydrogen phosphate) and acetonitrile in the ratio 60:40 (v/v). The flow rate
was 1 ml/min and detection was carried out by photodiode array detector at 268 nm. The retention time for DOXY was found
to be 2-897 min. The proposed method has permitted the quantification of DOXY over linearity in the range of 25 – 150
µg/ml and its percentage recovery was found to be 99.134 – 101.997 %. The % RSD of intraday and inter day precision were
found 0.7999% and 1.3%.

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Published

2014-12-03

How to Cite

Jeyabaskaran.M, Rambabu.C, Sree Janardhanan V, Rajinikanth.V, Pranitha.T, & Dhanalakshmi.B. (2014). RP-HPLC method development and validation of doxycycline in bulk and tablet formulation. IJPAR JOURNAL, 3(4), 397–404. https://doi.org/10.61096/ijpar.v3.iss4.2014.397-404

Issue

Vol. 3 No. 4 (2014): 2014 Volume - 3 Issue - 4

Section

Articles