LC method development and validation of aspirin and clopidogrel in pure API’S and its pharmaceutical dosage forms

Abstract

A simple, sensitive and precise reverse phase high performance liquid chromatographic method has been developed for
the estimation of Aspirin and Clopidogrel in pure sample and its pharmaceutical dosage form. The mobile phase consists
of Phosphate buffer (0.02 M Potassium dihydrogen phosphates, pH-3 adjusted with ortho phosphoric acid): acetonitrile
in the ratio of 65:35 v/v delivered at a flow rate of 1.0 ml / min and wavelength of detection at 229 nm. The retention
times of Aspirin and Clopidogrel were 2.673 and 3.627min respectively. The developed method was validated according
to ICH guidelines. The result indicates that the method was found to be simple, rapid, and accurate and can be adopted
in routine analysis of Aspirin and Clopidogrel in Pure sample and its Pharmaceutical dosage forms.