A new reverse phase HPLC method for quick quantificationof all four isomers of natural vitamin-E oil in a single analysis.

Abstract

A simple and fast analytical method for quantification of natural vitamin E isomers by reverse phase High Performance
Liquid Chromatography within short time. This method is developed to quantify in four natural isomers of vitamin- E,
namely alpha, beta, gamma and delta tocopherol in fine formulations. The four isomers elution was carried out by using
simple short column (100 X 4.6 mm) were used. And even mobile phase has used only Acetonitrile and water. The Limit of
detection was achieved of isomers are very lower levels like 3 ppm. The method is capable to quantify 10 ppm to 100 % in a
sample. The method is reliable robust, accurate and linear.