DEVELOPMENT AND VALIDATION OF UV-SPECTROPHOTOMETRIC METHOD FOR ESTIMATION OF ABACAVIR SULFATE NANOPARTICLES.

Abstract

A simple, precise and accurate spectrophotometric method has been developed for the determination of
Abacavir Sulfate nanoparticles. Standard Stock solution was prepared in pH 7.4 phosphate buffer and further
dilution were carried out with the same. The λmax of Abacavir Sulfate nanoparticles was found to be 285 nm.
The A (1%, 1cm) Value of Abacavir Sulfate nanoparticles was found to be 434. The correlation co efficient (r)
was found to be 0.9999. The limit of detection (LOD) and limit of Quantification (LOQ) was found to be 0.1074
μg/ml and 0.3257 μg/ml respectively for Abacavir Sulfate nanoparticles. The result of estimation in Abacavir
sulfate nanoparticles was found to be 99.62 ± 0.25%. The method was then validated statistically as per KH
guidelines, which yielded good results concerning range, linearity, precision, accuracy specifically, Robustness
and Ruggedness.