Formulation & in vitro evaluation of amphotericin B using β-cyclodextrin capped silver nanoparticles

Abstract

The prepared polymeric silver nanoparticles so formed were evaluated for %Entrapment efficiency, and in vitro release profiles. The in vitro drug release studies were carried out with apparatus (USP II) with paddle. The formulation  A7  showed  a drug release of 94.68% for 30mins  indicates  the increased  bioavailability of the drug.The formulation [A7] was selected as best formulation based on % Entrapment efficiency and drug release and  was subjected  to determination  of particle  size and  zeta  potential,  particle  morphology,  in-vitro  release studies. The formulation (A7] had particle size of 167.8 nm and the zeta potential (ζ) is -23.7 mV. The particle morphology of Amphotericin B loaded NPs was confirmed by Scanning Electron Microscope. Stability studies performed  for optimized  Amphotericin  B loaded  AgNPs  formulations  indicate  that it  have more stability at room temperature. The optimized formulation A7 follows Zero order kinetics with Higuchi.