Analytical method development and validation of levosulpride and pantoprazole by using reverse phase HPLC method

Abstract

A simple and precise method was developed for estimating levosulpride well as pantoprazole. The method was found to be specific and precise. The separation was attained on Hypersil BDS (100x4.6mm ID) 5.0µm column and linearity was achieved in the concentration range of 15µg/ml to 45µg/ml of Levosulpride, 8µg/ml to 24µg/ml of Pantoprazole with correlation coefficient 0.99. The percent recovery from the assay was found to be 99.8% for levosulpride and 99.6% for pantoprazole. Limit of detection and quantitation for levosulpride and pantoprazole were within the acceptable range. From the stability studies, the percentage variation was less than 10.0% which is the desired criteria. Therefore, this method can be adopted to estimate levosulpride as well as pantoprazolein other pharmaceutical formulations.