Method Development and Validation of Naftopidil by Reverse Phase- HPLC in Bulk and Pharmaceutical Dosage Forms

Authors

  • V.Gayathri Department of Pharmaceutical Analysis, Smt. Sarojini Ramulamma College of Pharmacy, Palamuru University, Mahaboobnagar, Andhra Pradesh.
  • M.Bhaskar Department of Pharmaceutical Analysis, Smt. Sarojini Ramulamma College of Pharmacy, Palamuru University, Mahaboobnagar, Andhra Pradesh.

DOI:

https://doi.org/10.61096/ijpar.v1.iss1.2012.29-34

Keywords:

Naftopidil, RP-HPLC, Validation, Methanol, ACN.

Abstract

A simple, sensitive accurate precise and rapid RP-HPLC  method was developed for the estimation of Naftopidil in bulk and pharmaceutical  formulation. The chromatographic  conditions used for separation was Kromosil C18 (150×4.6mm,  3.5µ) and the mobile  phase comprised  of acetonitrile  and water in the ratio of 50:50 %(v/v %) The flow  rate  was  1ml/min  with  the detection  at 232nm.The  retention  time  was  found  to be 3.245min.The proposed  method  is accurate  .The  linearity  was  found  to be in the  range  of 50-150  µg/ml  with  correlation coefficient  of  1.  The  repeatability  and  intermediate  precision  was  found  to  be less  than  2%.  The  Limit  of Quantitation  (LOQ)  were found to be 1.13 µg/ml  and 3.43 µg/ml respectively.  The method  was successfully applied to Pharmaceutical  formulation.

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Published

2012-12-20

How to Cite

V.Gayathri, & M.Bhaskar. (2012). Method Development and Validation of Naftopidil by Reverse Phase- HPLC in Bulk and Pharmaceutical Dosage Forms. IJPAR JOURNAL, 1(1), 29–34. https://doi.org/10.61096/ijpar.v1.iss1.2012.29-34

Issue

Vol. 1 No. 1 (2012): 2012 Volume - 1 Issue - 1

Section

Articles