Method development, validation and forced degradation study of serratiopeptidase by uv spectrometric method

Abstract

The pharmaceutical analysis is a branch of chemistry, which involves the series of process for the identification, determination, quantitation, and purification. The sample solution of 10 µg/mL of std stock solution of serratiopeptidase in distilled water prepared and the solution was scanned in UV region in the wavelength range from 200-400 nm by using distilled water as a solvent. The overlay spectra of serratiopeptidase was recorded. From the spectra, serrattiopeptidase shows maximum absorbance at 263 nm. Percentage of RSD for intraday and inter day precision studies for the drug was well within the acceptable range (<2%) indicating that the method have excellent repeatability and reproducibility. The percentage relative standard deviation for precision and accuracy was found to be low, which indicates that the method have considerable accuracy and precision. Percent recovery for serrattiopeptidase was found in the range of 99.87% to 100.71% with standard deviation well below two indicating accuracy of the method. Recovery greater than 98% with low standard deviation justifies the accuracy of the method. Intraday and inter day precision studies were carried out  by analyzing  tablet  formulation  by this method. The system suitability was found to meet the pre-established criteria at all the conditions and the degradation shows in acid (1.39%), alkaline (1.88%) peroxide (2.88%), thermal (1.14%), water (0.15%).