Method Development and Validation For The Simultaneous Estimation of Esomeprazole and Levosulpiride By Using RPHPLC In Its Bulk And Pharmaceutical Dosage Form

Abstract

Esomeprazole is a compound that inhibits gastric acid secretion and is indicated in the treatment of gastroesophageal reflux disease (GERD), the healing oferosive esophagitis, and H. pylori eradication to reduce the risk of duodenal ulcerrecurrence. Esomeprazole and Levosulpiride selective dopamine D2antagonist with antipsychotic and antidepressant activity.Asimple,Accurate, precise method was developed for the simultaneous estimation of the Esomeprazole and Levosulpiride in Tablet dosage form. Retentiontime of Esomeprazole and Levosulpiride were found to be 2.2min and 4.0min. % RSD of the Esomeprazole and Levosulpiride were and found to be 0.97 and 0.50 respectively. % Recover was Obtained as 100.08% and 101.16% for Esomeprazole and Levosulpiride respectively. LOD, LOQ values were obtained from regression equations of Esomeprazole and Levosulpiride were 0.10ppm, 0.34ppm and 0.29ppm, 1.04ppm respectively. Regression equation of Esomeprazole is y = 10568x + 307.3 and of Levosulpiride is y = 11649.x+ 1207. Retention times are decreased and thatrun time was decreased so the method developed was simple and economical that can be adopted in regular Quality control test in Industries.