Simultaneous estimation of new analytical method development and validation of pantoprazole, itopride hydrochloride by high performance liquid chromatography

Authors

  • Relangi Lokesh Siva Surya Gowd Department of Pharmaceutical Analysis, Pydah College of Pharmacy Patavala, Andhra University, Kakinada, Andhra Pradesh
  • T.K.V. Kesav Rao Department of Pharmaceutical Analysis, Pydah College of Pharmacy Patavala, Andhra University, Kakinada, Andhra Pradesh
  • A.Venkateswara Rao Department of Pharmaceutical Analysis, Pydah College of Pharmacy Patavala, Andhra University, Kakinada, Andhra Pradesh

Keywords:

Pantoprazole, Itopride Hydrochloride, RP-HPLC, Validation, Precision

Abstract

A rapid and precise reverse phase high performance liquid chromatographic method has been developed for the validated of Pantoprazole, Itopride Hydrochloride, in its pure form as well as in tablet dosage form. Chromatography was carried out on X bridge C18 (4.6×150mm) 5µcolumn using a mixture of Methanol: Phosphate Buffer pH3 (60:40v/v) as the mobile phase at a flow rate of 1.0ml/min, the detection was carried out at 260nm. The retention time of the Pantoprazole, Itopride Hydrochloride was 2.6, 3.8±0.02min respectively. The method produce linear responses in the concentration range of 5-25µg/ml of Pantoprazole and 20-100µg/ml of Itopride Hydrochloride respectively. The method precision for the determination of assay was below 2.0%RSD. The method is useful in the quality control of bulk and pharmaceutical formulations.

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Published

2023-08-15

How to Cite

Relangi Lokesh Siva Surya Gowd, T.K.V. Kesav Rao, & A.Venkateswara Rao. (2023). Simultaneous estimation of new analytical method development and validation of pantoprazole, itopride hydrochloride by high performance liquid chromatography. IJPAR JOURNAL, 12(3), 386–393. Retrieved from https://ijpar.com/ijpar/article/view/706

Issue

Vol. 12 No. 3 (2023): 2023 Volume - 12 Issue - 3

Section

Articles