Reverse phase-hplc method development and validation for the quantitative estimation of brivaracetam in bulk form and marketed pharmaceutical dosage form
Keywords:Brivaracetam, RP-HPLC, Validation, Accuracy, ICH Guidelines
A simple, rapid, specific and accurate reverse phase high performance liquid chromatographic method has been developed for the validated of Brivaracetam in bulk as well as in marketed pharmaceutical dosage form. This separation was performed on a Symmetry ODS C18 (4.6×250mm, 5µm) column with Methanol: Phosphate Buffer (pH-3.6 adjusted with OPA) (35:65%) v/v as mobile phase at a flow rate of 1.0 mL min−1 with UV detection at 235 nm; the constant column temperature was Ambient. The run time under these chromatographic conditions was less than 8 min. The retention time of Brivaracetam was found to be 2.286min. The calibration plot was linear over the concentration range of 6–14μg mL−1 with limits of detection and quantification values of 1.2 and 3.6 ng mL−1 respectively. The mean % assay of marketed formulation was found to be 99.86%, and % recovery was observed in the range of 98-102%. Relative standard deviation for the precision study was found <2%. The developed method is simple, precise, specific, accurate and rapid, making it suitable for estimation of Brivaracetam in bulk and marketed pharmaceutical dosage form dosage form.