Analytical Method Development And Validation For Simultaneous Estimation Trifluoperazine HCL, Trihexy Phenidylhcl In Combined Pharmaceutical Dosage Form By RP-HPLC
Keywords:Trihexyphenidyl HCL, Trifluoperazine HCL, RP-HPLC, Validation, Accuracy
A rapid and precise reverse phase high performance liquid chromatographic method has been developed for the validated of Trihexyphenidyl HCL and Trifluoperazine HCL, in its pure form as well as in tablet dosage form. Chromatography was carried out on an Altima C18 (4.6mm x 150mm, 5µm) column using a mixture of ACN, Methanol and Phosphate buffer pH-4.6 (10:25:65 v/v) as the mobile phase at a flow rate of 1.0ml/min, the detection was carried out at 265nm. The retention time of the Trihexyphenidyl HCL and Trifluoperazine HCL was 2.088, 6.068 ±0.02min respectively. The method produce linear responses in the concentration range of 10-50mg/ml of Trihexyphenidyl HCL and 20-100mg/ml of Trifluoperazine HCL. The method precision for the determination of assay was below 2.0%RSD. The method is useful in the quality control of bulk and pharmaceutical formulations.