Analytical method development and validation for the simultaneous estimation of azithromycin and levoflaxacin by RP-HPLC

Authors

  • Sanganamoni Tharun KGR Institute of Technology and Management, Rampally, affiliated to Osmania University, Hyderabad, Telangana, India-501301.
  • Gampa Vijay kumar KGR Institute of Technology and Management, Rampally, affiliated to Osmania University, Hyderabad, Telangana, India-501301.

DOI:

https://doi.org/10.61096/ijpar.v12.iss4.2023.637-646

Keywords:

Azithromycin, Levoflaxacin, RP HPLC, Validation Parameters

Abstract

A reverse phase high performance liquid chromatographic method was developed for simultaneous estimation of Azithromycin and Levoflaxacine. The chromatographic conditions were successfully developed for the separation of Azithromycin and Levoflaxacine by using ZODIAC –SIL RP C18 column 4.6×100 mm 3.0µm, flow rate was 1.0 ml/min, mobile phase ratio was (75:25 v/v) (KH 2 PO 4 and K 2 HPO 4 ) pH 9, detection wave length was 292 nm. The precision study was precise, robust, and repeatable. LOD value was 2.17 and 6.60, and LOQ value was 0.032 and 0.1125 respectively. Hence the suggested RP-HPLC method can be used for routine analysis of Azithromycin and Levoflaxacin in API and Pharmaceutical dosage form.

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Published

2023-12-01

How to Cite

Sanganamoni Tharun, & Gampa Vijay kumar. (2023). Analytical method development and validation for the simultaneous estimation of azithromycin and levoflaxacin by RP-HPLC. IJPAR JOURNAL, 12(4), 637–646. https://doi.org/10.61096/ijpar.v12.iss4.2023.637-646

Issue

Vol. 12 No. 4 (2023): 2023 Volume - 12 Issue - 4

Section

Articles