Development and Validation of an Extremely Analytical RP-HPLC Technique for the simultaneous estimation of Phentermine and Topiramate in API and mixed Marketed Pharmaceutical Dosage Forms
DOI:
https://doi.org/10.61096/ijpar.v10.iss1.2021.71-80Keywords:
Phentermine and Topiramate, RP-HPLC, Accuracy, ICH guidelinesAbstract
An accurate, unique and reproducible excessive-commonplace common ordinary performance liquid chromatographic technique for simultaneous estimation of Phentermine and Topiramate in bulk and tablet dosage paperwork. Chromatographic separations of the medicine were completed on a Symmetry ODS C18 (4.6×150mm, five.0 µm) the usage of a cellular segment together with Methanol: TEA Buffer pH-4.8 (35:sixty 5) v/v at a waft price of one.0 ml/min. the medicine elute had been monitored at 276 nm. The retention time received for the Phentermine grow to be 2.090 min and for the Topiramate come to be 5.289 min. The calibration curves had been linear over the form of 20-60µg/ml and 25-seventy-fiveµg/ml for Phentermine and Topiramate respectively. The method is showed as consistent with ICH guiding principle through figuring out its specificity, accuracy, precision, linearity & variety, ruggedness, robustness and tool suitability. The effects of the take a look at understanding knowledge that the proposed technique is easy, speedy, specific and accurate, that is useful for the habitual dedication of Phentermine and Topiramate in bulk and tablet dosage workplace paintings. The approach can be carried out for willpower of in its pill dosage office paintings with none interference from excipients or endogenous substances. The proposed approach is appropriate for habitual manage evaluation.