Development and Validation of an Extremely Analytical RP-HPLC Technique for the simultaneous estimation of Phentermine and Topiramate in API and mixed Marketed Pharmaceutical Dosage Forms

Authors

  • S.Spandana Department of Pharmaceutical analysis,, Holy Mary College of pharmacy, Keesara, ghatkesar,Telanagam 501301
  • Saikiran Department of Pharmaceutical analysis,, Holy Mary College of pharmacy, Keesara, ghatkesar,Telanagam 501301
  • Hemalatha Department of Pharmaceutical analysis,, Holy Mary College of pharmacy, Keesara, ghatkesar,Telanagam 501301
  • Ramya Sri.S Surya Pharma Labs, Dilsukhnagar, Hyderabad, Telangana-500060, India

DOI:

https://doi.org/10.61096/ijpar.v10.iss1.2021.71-80

Keywords:

Phentermine and Topiramate, RP-HPLC, Accuracy, ICH guidelines

Abstract

An accurate, unique and reproducible excessive-commonplace common ordinary performance liquid chromatographic  technique  for  simultaneous  estimation  of  Phentermine  and  Topiramate  in  bulk  and  tablet dosage paperwork. Chromatographic separations of the medicine were completed on a Symmetry ODS C18 (4.6×150mm, five.0 µm) the usage of a cellular segment together with Methanol: TEA Buffer pH-4.8 (35:sixty 5) v/v at a waft price of one.0 ml/min. the medicine elute had been monitored at 276 nm. The retention time received  for  the  Phentermine  grow  to  be  2.090  min  and  for  the  Topiramate  come  to  be  5.289  min.  The calibration curves had been linear over the form of 20-60µg/ml and 25-seventy-fiveµg/ml for Phentermine and Topiramate respectively. The method is showed as consistent with ICH guiding principle through figuring out its specificity, accuracy, precision, linearity & variety, ruggedness, robustness and tool suitability. The effects of  the  take  a  look  at  understanding  knowledge  that  the  proposed  technique  is  easy,  speedy,  specific  and accurate, that is useful for the habitual dedication of Phentermine and Topiramate in bulk and tablet dosage workplace paintings. The approach can be carried out for willpower of in its pill dosage office paintings with none interference from excipients or endogenous substances. The proposed approach is appropriate for habitual manage evaluation.

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Published

2022-08-26

How to Cite

S.Spandana, Saikiran, Hemalatha, & Ramya Sri.S. (2022). Development and Validation of an Extremely Analytical RP-HPLC Technique for the simultaneous estimation of Phentermine and Topiramate in API and mixed Marketed Pharmaceutical Dosage Forms. IJPAR JOURNAL, 10(1), 71–80. https://doi.org/10.61096/ijpar.v10.iss1.2021.71-80