Rp-Hplc Method Development And Validation For Estimation Of Thiocolchicoside And Aceclofenac In Pure And Pharmaceutical Dosage Form

Abstract

A simple, reproducible and efficient reverse phase high performance liquid chromatographic method was developed for simultaneous determination of Aceclofenac and Thiocolchicoside in pure form and marketed combined pharmaceutical dosage forms. A column having Symmetry (C18) (150mm x 4.6mm, 5µm) in isocratic mode with mobile phase containing Methanol: Phosphate Buffer (pH-3.8) (28:72% v/v) was used. The flow rate was 1.0 ml/min and effluent was monitored at 252 nm. The retention time (min) and linearity range (ppm) for Aceclofenac and Thiocolchicoside were (1.794, 3.440min) and (10-30, 10-50), respectively. The method has been validated for linearity, accuracy and precision, robustness and limit of detection and limit of quantitation. The limit of detection (LOD) and limit of quantification (LOQ) were found to be 0.86µg/ml and 2.58µg/ml for Aceclofenac and 1.28µg/ml 3.84µg/ml for Thiocolchicoside respectively. The developed method was found to be accurate, precise and selective for simultaneous determination of Aceclofenac and Thiocolchicoside in tablets.