In Vitro and In Vivo Evaluation of anti-diabetic Potential of Pisonia grandis

Abstract

The general interest in plants stems from their fundamental role in the commencement of life and their ability to produce various chemicals that act on the body, aiding in the treatment of diseases. The extraction of leaves from Pisonia grandis was conducted using various solvents with the Soxhlet apparatus. All extracts showed positive results in both in vitro and in vivo pharmacological studies. Our research findings indicate that for 50% inhibition of alpha-amylase activity, the concentrations were as follows: Ethyl acetate extract at 91 µg/ml, water extract at 80 µg/ml, petroleum ether extract at 75 µg/ml, and Acarbose (positive control) at 61 µg/ml. Our final report shows that the ethanolic extract has the highest anti-diabetic activity compared to the other extracts due to the presence of more anti-diabetic bioactive components. We conclude that this work will be useful in discovering novel entities for the treatment of diabetes mellitus.