A New Validated UV-spectroscopic Method for Simultaneous Estimation of Tenegliptin and Metformin in Pharmaceutical Dosage Form
DOI:
https://doi.org/10.61096/ijpar.v13.iss3.2024.376-381Keywords:
Simultaneous equation method, UV-spectrophotometer, Validation, Tenegliptin, Metformin HclAbstract
Simple, precise, economical, fast and reliable UV-Spectroscopic methods have been developed for the simultaneous estimation of Metformin HCl and Tenegliptin in bulk and pharmaceutical dosage form. The method which is based on measurement of absorption at maximum wavelength of 232 nm and 242 nm for Metformin HCl and Tenegliptin respectively. Linearity for detector response was observed in the concentration range of 2-10μg/ml for Metformin HCl and 2-10 μg/ml for Tenegliptin. The accuracy of the methods was assessed by recovery studies and was found to be 100.23 % and 99.36% for Metformin HCl and Tenegliptin respectively. The developed method was validated with respect to linearity, accuracy (recovery), precision and specificity. The results were validated statistically as per ICH Q2 R1 guideline and were found to be satisfactory. The proposed methods were successfully applied for the determination of for Metformin HCl and Tenegliptin in commercial pharmaceutical dosage form