Method Development And Validation For The Simultaneous Estimation Of Umeclidinium And Vilanterol In Solution Using UPLC Method

Abstract

A rapid and precise reverse phase high performance liquid chromatographic method has been developed for the validated of Umeclidinium and Vilanterol, in its pure form as well as in powder dosage form. Chromatography was carried out on Acquity UPLC BEH C-18,50 mm × 2.1 mm and 1.7 μm μm column using a mixture of Water and Acetonitrile (60:40% v/v) as the mobile phase at a flow rate of 0.9ml/min, the detection was carried out at 220nm. The retention time of the Umeclidinium and Vilanterol was 3.0, 3.8±0.02min respectively. The method produce linear responses in the concentration range of 5-25µg/ml of Umeclidinium and 10- 50µg/ml of Vilanterol. The method precision for the determination of assay was below 2.0%RSD. The method is useful in the quality control of bulk and pharmaceutical formulations.