Synthesis, Characterisation And Biological Evaluation Of Selenium Conjugated Naringenin Nanoparticles

Abstract

In this study, Selenium Nanoparticles (Se-N-NPs) were synthesized by chemical reduction method using sodium salt of selenium and ascorbic acid, a reducing agent and a bioactive molecule naringenin, a bioflavonoid from citrus fruits. The sodium selenite and ascorbic were mixed together followed by conjugation with naringenin. Se-N-NPs were characterized by UV, FTIR, SEM, TEM, EDAX, X-RD and Zeta Sizer analysis. The UV spectrum indicated the highest peak of absorbance at 293 nm in acetone whereas FTIR analysis of Se-N-NPs indicated absorbance bands at 3336 cm^-1 for OH group, 2926cm^-1 for CH₃ and 1631 cm^-1 C=O group. Drug release studies proved that the NPs release the drug in sustained manned with time. The antioxidant activity of NPs was carried by DPPH method using ascorbic acid as std.. The anticancer activity was studied as MCF-7 cancer cell line by MTT assay method. The obtained result showed that NPs have IC₅₀ 260μg which proved anticancer effect of NPs. Apoptosis studies were done with dual dye AO/EB stain. This result proved that the Se-N-NPs inhibited cancer cell growth by inducing apoptosis. Comprehensively it can be concluded that Se-N-NPs can be attempted to develop a nano formulation with anti-cancer potential. However, extensive research is needed to reach a concrete conclusion.