A Review On Rp-Hplc

Abstract

Chromatography is largely used in chemical analysis, where High- performance liquid chromatography (HPLC), a very flexible approach, is used to separate analytes by passing them through a column filled with micrometer-sized particles. Chromatography is essentially a separation technique. Now a days, the most popular separation method in HPLC is reversed-phase chromatography. This is due to the reversed phase method's ease of use, adaptability, and range, which allow it to handle compounds with a wide range of molecular masses and polarities. In the field of biological separation and purification, reversed phase chromatography has been used for both analytical and preparative purposes. Proteins, peptides, and nucleic acids are examples of molecules with some degree of hydrophobicity that can be separated using reversed phase chromatography with good recovery and resolution. In addition to providing a brief overview of the crucial chromatographic parameters that must be optimized for an effective method development, this paper discusses the significance of RP-HPLC in analytical method development and their techniques.