Uv spectrophotometric method development for the estimation of multiple antineoplastic agents : a comprehensive analytical study

Abstract

In terms of analytical instruments, UV-VIS spectroscopy was among the first. UV-Vis spectroscopy is a useful tool for characterizing a wide range of materials. Based on the different responses of samples and the degree of absorption or transmission of a range of beam light wavelengths, the UV-Vis provides information. It can be applied to qualitative as well as quantitative tests. Between 200 and 700 nm is the UV range. Additionally, the UV/Vis spectrum can help clarify the complexation mechanism involving templates, monomers, and cross-linkers during polymerization. This characterization procedure is rapid, easy, and reasonably priced. Many drugs are analysed by this method. Imatinib functions by blocking protein tyrosine kinases, especially BCR-ABL, which is constitutively activated in malignancies such as chronic myeloid leukemia (CML). Cisplatin mainly entails DNA damage, which kills cells. Through its binding to purine nucleotides in DNA, it creates crosslinks that obstruct transcription and DNA replication.Vincristine arrest cells in the metaphase, acting as antimicrotubule agents to prevent mitosis.