Geographical Variation Studies of Emblica officinalis Using Gallic Acid as Analytical Marker by HPLC Technique

Authors

  • S.Manimaran Department of Pharmacognosy, P.G.P.College of Pharmaceutical Science and Research Institute, Namakkal, Tamilnadu, India.
  • S.Kokila Vani Department of Pharmacognosy, P.G.P.College of Pharmaceutical Science and Research Institute, Namakkal, Tamilnadu, India.
  • P.Jeevan Prasath Department of Pharmacognosy, P.G.P.College of Pharmaceutical Science and Research Institute, Namakkal, Tamilnadu, India.
  • C.Manojkumar Department of Pharmacognosy, P.G.P.College of Pharmaceutical Science and Research Institute, Namakkal, Tamilnadu, India.
  • C.Singaravelan Department of Pharmacognosy, P.G.P.College of Pharmaceutical Science and Research Institute, Namakkal, Tamilnadu, India.
  • L.Monisha Department of Pharmacognosy, P.G.P.College of Pharmaceutical Science and Research Institute, Namakkal, Tamilnadu, India.
  • G.Arunachalam Department of Pharmacognosy, P.G.P.College of Pharmaceutical Science and Research Institute, Namakkal, Tamilnadu, India.
  • R. Joseph Sahaya Raja Synthiya Research Labs Pvt. Ltd., Pondicherry
  • R.Prasath Synthiya Research Labs Pvt. Ltd., Pondicherry

Keywords:

HPLC Analysis, Emblica officinalis, Raw material, Gallic acid.

Abstract

Standardized extracts are high quality extracts containing constituents levels of specified compounds and they are subjected to rigorous quality control during all phases of the growing, harvesting and manufacturing process. The active principles are unknown marker substances should be established for analytical purpose and standardization. The standardization of herbal raw material has become very essential as there is increase in the demand and frequent usage of the herbal raw materials. The herbal raw material containing Emblica officinalis fruits were collected from the  various geographical sources and  standardized for  their  gallic  acid  content by  High  Performance Liquid Chromatographic Technique (HPLC) is our objective of present study. The collected fresh fruits were cut in to small pieces, dried under shade and made to fine powder. The powdered raw materials were subjected to HPLC analysis to estimate the gallic acid content. The percentage of gallic acid was estimated by comparing the peak area of standard and the same present in the samples. The results reveal that there are lots of variations between the samples and the percentage of gallic acid is not uniform in all the collected samples. To get the good quality herbal products in the market the Good Agricultural and Collection Practices (GACP) to be maintained throughout the cultivation of medicinal plants to ensure the quality and uniform content of phytoconstituents which is responsible for the therapeutic activity.

Downloads

Published

2022-08-29