Geographical Variation Studies of Emblica officinalis Using Gallic Acid as Analytical Marker by HPLC Technique
Keywords:HPLC Analysis, Emblica officinalis, Raw material, Gallic acid.
Standardized extracts are high quality extracts containing constituents levels of specified compounds and they are subjected to rigorous quality control during all phases of the growing, harvesting and manufacturing process. The active principles are unknown marker substances should be established for analytical purpose and standardization. The standardization of herbal raw material has become very essential as there is increase in the demand and frequent usage of the herbal raw materials. The herbal raw material containing Emblica officinalis fruits were collected from the various geographical sources and standardized for their gallic acid content by High Performance Liquid Chromatographic Technique (HPLC) is our objective of present study. The collected fresh fruits were cut in to small pieces, dried under shade and made to fine powder. The powdered raw materials were subjected to HPLC analysis to estimate the gallic acid content. The percentage of gallic acid was estimated by comparing the peak area of standard and the same present in the samples. The results reveal that there are lots of variations between the samples and the percentage of gallic acid is not uniform in all the collected samples. To get the good quality herbal products in the market the Good Agricultural and Collection Practices (GACP) to be maintained throughout the cultivation of medicinal plants to ensure the quality and uniform content of phytoconstituents which is responsible for the therapeutic activity.