Quantitative Estimation Of Sofosbuvir And Velpatasvir In Tablet Dosage Forms By Rp-Hplc Method

Abstract

A rapid and precise Reverse Phase High Performance Liquid Chromatographic method has been developed for the validated of Sofosbuvir and Velpatasvir, in its pure form as well as in tablet dosage form. Chromatography was carried out on a Symmetry C18 (4.6 x 150mm, 5µm) column using a mixture of Methanol: TEA pH 4.2 (40:60) as the mobile phase at a flow rate of 1.0ml/min, the detection was carried out at 272 nm. The retention time of the Sofosbuvir and Velpatasvir was 2.781, 4.048 ±0.02min respectively. The method produce linear responses in the concentration range of 7.5-37.5µg/ml of Sofosbuvir and 5-25µg/ml of Velpatasvir. The method precision for the determination of assay was below 2.0%RSD. The method is useful in the quality control of bulk and pharmaceutical formulations.